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Pediatrics Hematology/Oncology - Project Scientist series - Nowicki Lab
University of California Los Angeles
Details
Posted: 30-Jan-26
Location: Los Angeles, California
Type: Full-time
Categories:
Academic/Faculty
Medical - Research
Internal Number: 6888586
University of California Los Angeles
Requisition Number: JPF10807
The Division of Hematology-Oncology in the Department of Pediatrics of the David Geffen School of Medicine at UCLA is seeking a Project Scientist. The appointment will be in the Project Scientist series; academic rank will be commensurate with experience.
The position requires a Ph.D. or equivalent and prior experience. This is a non-senate position.
Duties and Responsibilities include:
- Manufacturing products for clinical research according to Good Manufacturing Practice (GMP) guidelines
- Operate in a GMP regulated environment according current standard operating protocols
- Meticulous record keeping and maintenance of GMP, GTP and basic research related records and documents.
- Attend and arrange seminars, training sessions and workshops to maintain competency in evolving current GMP/GTP regulations
- Performing post-manufacturing lot-release tests: Elisa, Colony-forming Unit (CFU) assay, ELISA, Endotoxin assay, Mycoplasma assay, vector copy number (VCN) test by ddPCR
- Culture of hematopoietic progenitor cells for in vitro differentiation into T-cells for proof of principle testing of gene delivery
- Conduct experiments for FDA IND application(s)
- Conduct DNA, RNA and protein isolation and purification according current GLP research grade protocols
- Quantification of nucleic acids and proteins
- Perform PCR and qPCR
- Perform multiparametric flow cytometry to assist on-going specific NYESO-TCR research project for intracellular, surface and peptide target markers.
- Culture adhered and non-adherent mammalian cells, preparing media, aliquots and other compounds utilized in tissue culture with proper sterile aseptic technique.
- Ability to set up co-cultures for killing-assays or peptide release production-analysis
- Ability to set up experiments utilizing real time in vitro cell imaging system Incucyte
- Perform ELISA experiments in either cellular supernatants or cellular lysates normalizing results against total protein content
- Plasmid preparation, bacteria growth on competent E. coli or Stable 3 systems, positive transformants selection and sequences analysis
- Ability of align sequences on APE-software (or-equivalent) and design plasmids for plasmid-vector construction and PCR analysis
- Perform plasmid sequencing and re-sequencing
- Production of high-titer viral vectors, purification and concentration of viral supernatants for retrovirus
- Production of high-titer viral vectors, purification and concentration of viral supernatants for lentivirus
- Determination of viral titer by ddPCR on fresh and frozen supernatants
- Document results of experiments and elaboration of reports for NYESO-TCR team leader
- Perform experiments associated to survival tittering and transduction efficiency on target cells
- Design and re-design of viral vectors for large scale manufacture of GMP and GMP-comparable clinical grade virus.
- Performs other job-related duties as assigned
Interested candidates can apply online at: https://recruit.apo.ucla.edu/JPF10807
UC Regents Statement on Ethical Values and Standards of Conduct:
All aspects of searches are confidential and all candidates are expected to review and abide by UC Regents Policy 1111 on Statement on Ethical Values and Standards of Conduct https://regents.universityofcalifornia.edu/governance/policies/1111.html
Cultural North Star:
The shared values of the DGSOM are expressed in the Cultural North Star, which was developed by members of our community and affirms our unswerving commitment to doing what's right, making things better, and being kind. These are the standards to which we hold ourselves, and one another. Please read more about this important DGSOM program at https://medschool.ucla.edu/cultural-north-star.
To apply, please visit: https://recruit.apo.ucla.edu/JPF10807
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy, see: UC Nondiscrimination & Affirmative Action Policy, https://policy.ucop.edu/doc/4000376/DiscHarassAffirmAction
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UCLA is known worldwide for the breadth and quality of its academic, research, health care, cultural, continuing education and athletic programs. UCLA offers undergraduate degrees in more than 127 majors and graduate degrees in 198 program areas. UCLA has 11 highly regarded professional schools. Eight are ranked among the nation's top 15 in their field by U.S. News & World Report. UCLA is consistently among the most popular campus in the nation for undergraduate applicants. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to age, race, color, religion, sex, national origin, disability, or protected veteran status. Benefits:UCLA offers a comprehensive benefits package, including an average of three weeks' vacation per year; an average of 12 days per year sick leave; 13 paid holidays per year; health, dental and optical benefits; life insurance; disability insurance; the University of California Retirement Plan with 5 year vesting and various voluntary UC Savings Plans. There are also special programs and privileges available, such as accessibility to cultural and recreational programs, athletic events, and the University Credit Union.
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https://main.hercjobs.org/jobs/22011214/pediatrics-hematology-oncology-project-scientist-series-nowicki-lab
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